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cas9n plasmid  (Addgene inc)


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    Addgene inc cas9n plasmid
    Cas9n Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psmvp+cas9n/pSMVP-Cas9N+(Plasmid+%2380934)/pmc10853780-65-7-15
    Average 91 stars, based on 7 article reviews
    cas9n plasmid - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    other:

    Article Title: mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy.
    Article Snippet: The pAAV.CMV.Luc.IRES.EGFP.SV40, dCas9-VPR, pSMVP-Cas9N, pSMVP-Cas9C, pAAV-CMV-Cas9C-VPR, and pCMV-PE2 plasmids were obtained from Addgene (#105533, #63798, #80934, #80939, and #80933, #132775).

    Article Title: mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy
    Article Snippet: The pAAV.CMV.Luc.IRES.EGFP.SV40, dCas9-VPR, pSMVP-Cas9N, pSMVP-Cas9C, pAAV-CMV-Cas9C-VPR, and pCMV-PE2 plasmids were obtained from Addgene (#105533, #63798, #80934, #80939, and #80933, #132775).

    Article Title: mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy
    Article Snippet: The pAAV.CMV.Luc.IRES.EGFP.SV40, dCas9-VPR, pSMVP-Cas9N, pSMVP-Cas9C, pAAV-CMV-Cas9C-VPR, and pCMV-PE2 plasmids were obtained from Addgene (#105533, #63798, #80934, #80939, and #80933, #132775).

    Cloning:

    Article Title: A gene therapy for inherited blindness using dCas9-VPR–mediated transcriptional activation
    Article Snippet: .. The Cas9m4-VP64, SP-dCas9-VPR, pSMVP-Cas9N, pSMVP-Cas9C, and pAAV-CMV-Cas9C-VPR plasmids were obtained from Addgene (#47319, #63798, #80934, #80939, and #80933, respectively). sgRNAs expressed via a U6 promoter were added using standard cloning techniques. .. Cas9N and Cas9C were rendered catalytically inactive by introducing the D10A and the H840A point mutations via a standard site-directed mutagenesis protocol using the KAPA HiFi HotStart ReadyMix PCR kit (Kapa Biosystems).



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    cas9n plasmid  (Addgene inc)
    91
    Addgene inc cas9n plasmid
    Cas9n Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psmvp+cas9n/pSMVP-Cas9N+(Plasmid+%2380934)/pmc10853780-65-7-15
    Average 91 stars, based on 1 article reviews
    cas9n plasmid - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    psmvp cas9n  (Addgene inc)
    91
    Addgene inc psmvp cas9n
    Psmvp Cas9n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psmvp+cas9n/pSMVP-Cas9N+(Plasmid+%2380934)/pm37852949-259-3-12
    Average 91 stars, based on 1 article reviews
    psmvp cas9n - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    cas9n  (Addgene inc)
    91
    Addgene inc cas9n
    Brk expression induction in response to Taxol treatment or suspension culture stress ULA is HIF dependent. MDA-MB-231 cells expressing the control empty vector, pX462-puromcyin (px462) or in which HIF-1α and HIF-2α were deleted via <t>CRISPR-Cas9n-mediated</t> gene editing (DKO) cells were treated with either vehicle or 10 nM Taxol for 24 h. (A) Brk protein levels were compared by western blotting with antibodies for Brk and p38 MAPK (loading control) and (B) mRNA expression was measured by following normalization to TBP expression. (C) MDA-MB-231 pX462 control cells or HIF-DKO cells were cultured on either adherent dishes (Att) or as single cells in ULA dishes for 24 h and assessed by western blotting with Brk and p38 MAPK (loading control) antibodies or (D) by qRT-PCR analysis to determine Brk mRNA expression levels relative to TBP expression. Statistical significance was assessed by the unpaired Student’s t-test (*, P < 0.05, **, P < 0.01, ***, P < 0.001).
    Cas9n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psmvp+cas9n/pSMVP-Cas9N+(Plasmid+%2380934)/pmc06214723-45-17-18
    Average 91 stars, based on 1 article reviews
    cas9n - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

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    Brk expression induction in response to Taxol treatment or suspension culture stress ULA is HIF dependent. MDA-MB-231 cells expressing the control empty vector, pX462-puromcyin (px462) or in which HIF-1α and HIF-2α were deleted via CRISPR-Cas9n-mediated gene editing (DKO) cells were treated with either vehicle or 10 nM Taxol for 24 h. (A) Brk protein levels were compared by western blotting with antibodies for Brk and p38 MAPK (loading control) and (B) mRNA expression was measured by following normalization to TBP expression. (C) MDA-MB-231 pX462 control cells or HIF-DKO cells were cultured on either adherent dishes (Att) or as single cells in ULA dishes for 24 h and assessed by western blotting with Brk and p38 MAPK (loading control) antibodies or (D) by qRT-PCR analysis to determine Brk mRNA expression levels relative to TBP expression. Statistical significance was assessed by the unpaired Student’s t-test (*, P < 0.05, **, P < 0.01, ***, P < 0.001).

    Journal: Molecular cancer research : MCR

    Article Title: Taxol Induces Brk-dependent Pro-survival Phenotypes in TNBC Cells through an AhR/GR/HIF-driven Signaling Axis

    doi: 10.1158/1541-7786.MCR-18-0410

    Figure Lengend Snippet: Brk expression induction in response to Taxol treatment or suspension culture stress ULA is HIF dependent. MDA-MB-231 cells expressing the control empty vector, pX462-puromcyin (px462) or in which HIF-1α and HIF-2α were deleted via CRISPR-Cas9n-mediated gene editing (DKO) cells were treated with either vehicle or 10 nM Taxol for 24 h. (A) Brk protein levels were compared by western blotting with antibodies for Brk and p38 MAPK (loading control) and (B) mRNA expression was measured by following normalization to TBP expression. (C) MDA-MB-231 pX462 control cells or HIF-DKO cells were cultured on either adherent dishes (Att) or as single cells in ULA dishes for 24 h and assessed by western blotting with Brk and p38 MAPK (loading control) antibodies or (D) by qRT-PCR analysis to determine Brk mRNA expression levels relative to TBP expression. Statistical significance was assessed by the unpaired Student’s t-test (*, P < 0.05, **, P < 0.01, ***, P < 0.001).

    Article Snippet: Each gRNA to target exon 1 of HIF1A was individually cloned into the pX462-puromycin vector, which expresses Cas9n (AddGene). pX462 was also modified to express hygromycin instead of puromycin and the HIF2A guides were individually cloned into pX462-hygromycin.

    Techniques: Expressing, Suspension, Control, Plasmid Preparation, CRISPR, Western Blot, Cell Culture, Quantitative RT-PCR